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p18 nfe2  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology p18 nfe2
    MMP9, CD147, cathepsin B, <t>p18</t> <t>NFE2,</t> p45 NFE2 and ADAMTSL-4 expression in ATII cells and lung tissue obtained from non-smokers (NS), smokers (SM) and patients with emphysema (E). MMP9, CD147 and cathepsin B expression in freshly isolated ATII cells ( A ) and lung tissue ( B ) as detected by Western blotting analysis. ( C ) MMP9, CD147, cathepsin B, p45 NFE2 and ADAMTSL-4 mRNA levels in lung tissue by RT-PCR. ( D,E ) p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in freshly isolated ATII cells ( D ) and lung tissue ( E ). Protein levels were analyzed by Western blotting. Densitometric analysis is also shown. *Statistically significant difference ( p < 0.05) is shown for comparison between non-smokers and smokers, between smokers and emphysema patients and between non-smokers and emphysema patients. Data are shown as the mean (±s.e.m.).
    P18 Nfe2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p18+nfe2/NF-E2+p18+Antibody/pmc05824795-191-17-6
    Average 93 stars, based on 20 article reviews
    p18 nfe2 - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema"

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-21790-3

    MMP9, CD147, cathepsin B, p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in ATII cells and lung tissue obtained from non-smokers (NS), smokers (SM) and patients with emphysema (E). MMP9, CD147 and cathepsin B expression in freshly isolated ATII cells ( A ) and lung tissue ( B ) as detected by Western blotting analysis. ( C ) MMP9, CD147, cathepsin B, p45 NFE2 and ADAMTSL-4 mRNA levels in lung tissue by RT-PCR. ( D,E ) p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in freshly isolated ATII cells ( D ) and lung tissue ( E ). Protein levels were analyzed by Western blotting. Densitometric analysis is also shown. *Statistically significant difference ( p < 0.05) is shown for comparison between non-smokers and smokers, between smokers and emphysema patients and between non-smokers and emphysema patients. Data are shown as the mean (±s.e.m.).
    Figure Legend Snippet: MMP9, CD147, cathepsin B, p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in ATII cells and lung tissue obtained from non-smokers (NS), smokers (SM) and patients with emphysema (E). MMP9, CD147 and cathepsin B expression in freshly isolated ATII cells ( A ) and lung tissue ( B ) as detected by Western blotting analysis. ( C ) MMP9, CD147, cathepsin B, p45 NFE2 and ADAMTSL-4 mRNA levels in lung tissue by RT-PCR. ( D,E ) p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in freshly isolated ATII cells ( D ) and lung tissue ( E ). Protein levels were analyzed by Western blotting. Densitometric analysis is also shown. *Statistically significant difference ( p < 0.05) is shown for comparison between non-smokers and smokers, between smokers and emphysema patients and between non-smokers and emphysema patients. Data are shown as the mean (±s.e.m.).

    Techniques Used: Expressing, Isolation, Western Blot, Reverse Transcription Polymerase Chain Reaction

    p45 NFE2 and p18 NFE2 tyrosine phosphorylation in human ATII cells and lung tissue obtained from control non-smokers (NS) and smokers (SM) and patients with emphysema (E). Immunoprecipitation was performed in freshly isolated ATII cells ( A ) and lung tissue ( B ) followed by Western blotting analysis. Densitometric quantification is also shown. *Statistically significant difference ( p < 0.05). Data are shown as the mean (±s.e.m.).
    Figure Legend Snippet: p45 NFE2 and p18 NFE2 tyrosine phosphorylation in human ATII cells and lung tissue obtained from control non-smokers (NS) and smokers (SM) and patients with emphysema (E). Immunoprecipitation was performed in freshly isolated ATII cells ( A ) and lung tissue ( B ) followed by Western blotting analysis. Densitometric quantification is also shown. *Statistically significant difference ( p < 0.05). Data are shown as the mean (±s.e.m.).

    Techniques Used: Immunoprecipitation, Isolation, Western Blot

    NFE2 knockdown increases cell death induced by CSE in A549 cells in vitro . ( A ) A549 cells were transfected with 100 nM NFE2 siRNA or non-targeting (NT) siRNA followed by exposure to CSE for 24 h. Representative flow cytometry images using Annexin V and PI staining are shown. ( B ) NFE2 knockdown significantly increased cell death after treatment with CSE compared to control. *Statistically significant difference ( p < 0.05). Data are shown as the mean (±s.e.m.).
    Figure Legend Snippet: NFE2 knockdown increases cell death induced by CSE in A549 cells in vitro . ( A ) A549 cells were transfected with 100 nM NFE2 siRNA or non-targeting (NT) siRNA followed by exposure to CSE for 24 h. Representative flow cytometry images using Annexin V and PI staining are shown. ( B ) NFE2 knockdown significantly increased cell death after treatment with CSE compared to control. *Statistically significant difference ( p < 0.05). Data are shown as the mean (±s.e.m.).

    Techniques Used: In Vitro, Transfection, Flow Cytometry, Staining

    p45 NFE2 interaction with DJ-1 in ATII cells and lung tissue obtained from non-smokers (NS), smokers (SM) and patients with emphysema (E). DJ-1 was co-immunoprecipitated in freshly isolated ATII cells ( A ) or lung tissue ( B ) followed by Western blotting analysis to determine its interaction with p45 NFE2 or p18 NFE2 as described in Materials and Methods. Relative expression is also shown. ( C ) p45 NFE2 (green) and DJ-1 (red) expression in ATII cells identified using SP-A staining (violet) in lung tissue by immunohistofluorescence. Cell nuclei were stained with DAPI (blue). The strongest co-localization of p45 NFE2 and DJ-1 is indicated by white arrows. *Statistically significant difference ( p < 0.05). Data are shown as the mean (±s.e.m.).
    Figure Legend Snippet: p45 NFE2 interaction with DJ-1 in ATII cells and lung tissue obtained from non-smokers (NS), smokers (SM) and patients with emphysema (E). DJ-1 was co-immunoprecipitated in freshly isolated ATII cells ( A ) or lung tissue ( B ) followed by Western blotting analysis to determine its interaction with p45 NFE2 or p18 NFE2 as described in Materials and Methods. Relative expression is also shown. ( C ) p45 NFE2 (green) and DJ-1 (red) expression in ATII cells identified using SP-A staining (violet) in lung tissue by immunohistofluorescence. Cell nuclei were stained with DAPI (blue). The strongest co-localization of p45 NFE2 and DJ-1 is indicated by white arrows. *Statistically significant difference ( p < 0.05). Data are shown as the mean (±s.e.m.).

    Techniques Used: Immunoprecipitation, Isolation, Western Blot, Expressing, Staining, Immunohistofluorescence

    MMP9, CD147, cathepsin B, p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in wild-type and DJ-1 KO mice. Wild-type (WT) ( A ) and DJ-1 KO mice ( B ) were exposed to 150 mg/m 3 cigarette smoke (CS) for 2 h/day for 3 weeks as described in Materials and Methods section. Protein levels were analyzed in lung tissue by Western blotting. Lane 1 – WT mice, Lane 2 – WT + CS, Lane 3 – DJ-1 KO mice, Lane 4 – DJ-1 KO mice + CS. Relative expression is also shown. ( C ) p45 NFE2 expression (green) in ATII cells identified using SP-A antibody (violet) in lung tissue obtained from wild-type and DJ-1 KO mice by immunohistofluorescence. Cell nuclei were stained with DAPI (blue). ( D , E ) MMP9, CD147, cathepsin B, p45 NFE2 and ADAMTSL-4 mRNA expression in lung tissue from wild-type ( D ) and DJ-1 KO ( E ) mice by RT-PCR. The strongest p45 NFE2 expression is indicated by white arrows. *Statistically significant difference in comparison with control ( p < 0.05). Data are shown as the mean (±s.e.m.).
    Figure Legend Snippet: MMP9, CD147, cathepsin B, p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in wild-type and DJ-1 KO mice. Wild-type (WT) ( A ) and DJ-1 KO mice ( B ) were exposed to 150 mg/m 3 cigarette smoke (CS) for 2 h/day for 3 weeks as described in Materials and Methods section. Protein levels were analyzed in lung tissue by Western blotting. Lane 1 – WT mice, Lane 2 – WT + CS, Lane 3 – DJ-1 KO mice, Lane 4 – DJ-1 KO mice + CS. Relative expression is also shown. ( C ) p45 NFE2 expression (green) in ATII cells identified using SP-A antibody (violet) in lung tissue obtained from wild-type and DJ-1 KO mice by immunohistofluorescence. Cell nuclei were stained with DAPI (blue). ( D , E ) MMP9, CD147, cathepsin B, p45 NFE2 and ADAMTSL-4 mRNA expression in lung tissue from wild-type ( D ) and DJ-1 KO ( E ) mice by RT-PCR. The strongest p45 NFE2 expression is indicated by white arrows. *Statistically significant difference in comparison with control ( p < 0.05). Data are shown as the mean (±s.e.m.).

    Techniques Used: Expressing, Western Blot, Immunohistofluorescence, Staining, Reverse Transcription Polymerase Chain Reaction

    The role of p45 NFE in ATII cells in non-smokers, smokers and emphysema patients.
    Figure Legend Snippet: The role of p45 NFE in ATII cells in non-smokers, smokers and emphysema patients.

    Techniques Used:

    Related Articles

    Expressing:

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema
    Article Snippet: GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).. The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.. Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).

    Isolation:

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema
    Article Snippet: GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).. The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.. Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).

    Western Blot:

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema
    Article Snippet: GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).. The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.. Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema
    Article Snippet: GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).. The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.. Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).

    Immunoprecipitation:

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema
    Article Snippet: GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).. The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.. Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).

    In Vitro:

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema
    Article Snippet: GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).. The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.. Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).

    Transfection:

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema
    Article Snippet: GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).. The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.. Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).

    Flow Cytometry:

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema
    Article Snippet: GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).. The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.. Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).

    Staining:

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema
    Article Snippet: GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).. The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.. Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).

    Immunohistofluorescence:

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema
    Article Snippet: GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).GAPDH and NRF2 antibodies were obtained from Abcam (Cambridge, MA) and β-actin was purchased from Sigma-Aldrich (St. Louis, MO).. The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.. Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).Lung tissue used for Western blotting was homogenized in Tissue PE-LB buffer (G-Biosciences, Saint Louis, MO) and ATII and A549 cells were lysed in Mammalian Cell PE buffer (G-Biosciences) with protease and phosphatase inhibitors (Gold Biotechnology, St. Louis, MO).



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    p18 nfe2  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology p18 nfe2
    MMP9, CD147, cathepsin B, <t>p18</t> <t>NFE2,</t> p45 NFE2 and ADAMTSL-4 expression in ATII cells and lung tissue obtained from non-smokers (NS), smokers (SM) and patients with emphysema (E). MMP9, CD147 and cathepsin B expression in freshly isolated ATII cells ( A ) and lung tissue ( B ) as detected by Western blotting analysis. ( C ) MMP9, CD147, cathepsin B, p45 NFE2 and ADAMTSL-4 mRNA levels in lung tissue by RT-PCR. ( D,E ) p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in freshly isolated ATII cells ( D ) and lung tissue ( E ). Protein levels were analyzed by Western blotting. Densitometric analysis is also shown. *Statistically significant difference ( p < 0.05) is shown for comparison between non-smokers and smokers, between smokers and emphysema patients and between non-smokers and emphysema patients. Data are shown as the mean (±s.e.m.).
    P18 Nfe2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p18+nfe2/NF-E2+p18+Antibody/pmc05824795-191-17-6
    Average 93 stars, based on 1 article reviews
    p18 nfe2 - by Bioz Stars, 2026-09
    93/100 stars
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    Image Search Results


    MMP9, CD147, cathepsin B, p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in ATII cells and lung tissue obtained from non-smokers (NS), smokers (SM) and patients with emphysema (E). MMP9, CD147 and cathepsin B expression in freshly isolated ATII cells ( A ) and lung tissue ( B ) as detected by Western blotting analysis. ( C ) MMP9, CD147, cathepsin B, p45 NFE2 and ADAMTSL-4 mRNA levels in lung tissue by RT-PCR. ( D,E ) p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in freshly isolated ATII cells ( D ) and lung tissue ( E ). Protein levels were analyzed by Western blotting. Densitometric analysis is also shown. *Statistically significant difference ( p < 0.05) is shown for comparison between non-smokers and smokers, between smokers and emphysema patients and between non-smokers and emphysema patients. Data are shown as the mean (±s.e.m.).

    Journal: Scientific Reports

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema

    doi: 10.1038/s41598-018-21790-3

    Figure Lengend Snippet: MMP9, CD147, cathepsin B, p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in ATII cells and lung tissue obtained from non-smokers (NS), smokers (SM) and patients with emphysema (E). MMP9, CD147 and cathepsin B expression in freshly isolated ATII cells ( A ) and lung tissue ( B ) as detected by Western blotting analysis. ( C ) MMP9, CD147, cathepsin B, p45 NFE2 and ADAMTSL-4 mRNA levels in lung tissue by RT-PCR. ( D,E ) p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in freshly isolated ATII cells ( D ) and lung tissue ( E ). Protein levels were analyzed by Western blotting. Densitometric analysis is also shown. *Statistically significant difference ( p < 0.05) is shown for comparison between non-smokers and smokers, between smokers and emphysema patients and between non-smokers and emphysema patients. Data are shown as the mean (±s.e.m.).

    Article Snippet: The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.

    Techniques: Expressing, Isolation, Western Blot, Reverse Transcription Polymerase Chain Reaction

    p45 NFE2 and p18 NFE2 tyrosine phosphorylation in human ATII cells and lung tissue obtained from control non-smokers (NS) and smokers (SM) and patients with emphysema (E). Immunoprecipitation was performed in freshly isolated ATII cells ( A ) and lung tissue ( B ) followed by Western blotting analysis. Densitometric quantification is also shown. *Statistically significant difference ( p < 0.05). Data are shown as the mean (±s.e.m.).

    Journal: Scientific Reports

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema

    doi: 10.1038/s41598-018-21790-3

    Figure Lengend Snippet: p45 NFE2 and p18 NFE2 tyrosine phosphorylation in human ATII cells and lung tissue obtained from control non-smokers (NS) and smokers (SM) and patients with emphysema (E). Immunoprecipitation was performed in freshly isolated ATII cells ( A ) and lung tissue ( B ) followed by Western blotting analysis. Densitometric quantification is also shown. *Statistically significant difference ( p < 0.05). Data are shown as the mean (±s.e.m.).

    Article Snippet: The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.

    Techniques: Immunoprecipitation, Isolation, Western Blot

    NFE2 knockdown increases cell death induced by CSE in A549 cells in vitro . ( A ) A549 cells were transfected with 100 nM NFE2 siRNA or non-targeting (NT) siRNA followed by exposure to CSE for 24 h. Representative flow cytometry images using Annexin V and PI staining are shown. ( B ) NFE2 knockdown significantly increased cell death after treatment with CSE compared to control. *Statistically significant difference ( p < 0.05). Data are shown as the mean (±s.e.m.).

    Journal: Scientific Reports

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema

    doi: 10.1038/s41598-018-21790-3

    Figure Lengend Snippet: NFE2 knockdown increases cell death induced by CSE in A549 cells in vitro . ( A ) A549 cells were transfected with 100 nM NFE2 siRNA or non-targeting (NT) siRNA followed by exposure to CSE for 24 h. Representative flow cytometry images using Annexin V and PI staining are shown. ( B ) NFE2 knockdown significantly increased cell death after treatment with CSE compared to control. *Statistically significant difference ( p < 0.05). Data are shown as the mean (±s.e.m.).

    Article Snippet: The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.

    Techniques: In Vitro, Transfection, Flow Cytometry, Staining

    p45 NFE2 interaction with DJ-1 in ATII cells and lung tissue obtained from non-smokers (NS), smokers (SM) and patients with emphysema (E). DJ-1 was co-immunoprecipitated in freshly isolated ATII cells ( A ) or lung tissue ( B ) followed by Western blotting analysis to determine its interaction with p45 NFE2 or p18 NFE2 as described in Materials and Methods. Relative expression is also shown. ( C ) p45 NFE2 (green) and DJ-1 (red) expression in ATII cells identified using SP-A staining (violet) in lung tissue by immunohistofluorescence. Cell nuclei were stained with DAPI (blue). The strongest co-localization of p45 NFE2 and DJ-1 is indicated by white arrows. *Statistically significant difference ( p < 0.05). Data are shown as the mean (±s.e.m.).

    Journal: Scientific Reports

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema

    doi: 10.1038/s41598-018-21790-3

    Figure Lengend Snippet: p45 NFE2 interaction with DJ-1 in ATII cells and lung tissue obtained from non-smokers (NS), smokers (SM) and patients with emphysema (E). DJ-1 was co-immunoprecipitated in freshly isolated ATII cells ( A ) or lung tissue ( B ) followed by Western blotting analysis to determine its interaction with p45 NFE2 or p18 NFE2 as described in Materials and Methods. Relative expression is also shown. ( C ) p45 NFE2 (green) and DJ-1 (red) expression in ATII cells identified using SP-A staining (violet) in lung tissue by immunohistofluorescence. Cell nuclei were stained with DAPI (blue). The strongest co-localization of p45 NFE2 and DJ-1 is indicated by white arrows. *Statistically significant difference ( p < 0.05). Data are shown as the mean (±s.e.m.).

    Article Snippet: The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.

    Techniques: Immunoprecipitation, Isolation, Western Blot, Expressing, Staining, Immunohistofluorescence

    MMP9, CD147, cathepsin B, p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in wild-type and DJ-1 KO mice. Wild-type (WT) ( A ) and DJ-1 KO mice ( B ) were exposed to 150 mg/m 3 cigarette smoke (CS) for 2 h/day for 3 weeks as described in Materials and Methods section. Protein levels were analyzed in lung tissue by Western blotting. Lane 1 – WT mice, Lane 2 – WT + CS, Lane 3 – DJ-1 KO mice, Lane 4 – DJ-1 KO mice + CS. Relative expression is also shown. ( C ) p45 NFE2 expression (green) in ATII cells identified using SP-A antibody (violet) in lung tissue obtained from wild-type and DJ-1 KO mice by immunohistofluorescence. Cell nuclei were stained with DAPI (blue). ( D , E ) MMP9, CD147, cathepsin B, p45 NFE2 and ADAMTSL-4 mRNA expression in lung tissue from wild-type ( D ) and DJ-1 KO ( E ) mice by RT-PCR. The strongest p45 NFE2 expression is indicated by white arrows. *Statistically significant difference in comparison with control ( p < 0.05). Data are shown as the mean (±s.e.m.).

    Journal: Scientific Reports

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema

    doi: 10.1038/s41598-018-21790-3

    Figure Lengend Snippet: MMP9, CD147, cathepsin B, p18 NFE2, p45 NFE2 and ADAMTSL-4 expression in wild-type and DJ-1 KO mice. Wild-type (WT) ( A ) and DJ-1 KO mice ( B ) were exposed to 150 mg/m 3 cigarette smoke (CS) for 2 h/day for 3 weeks as described in Materials and Methods section. Protein levels were analyzed in lung tissue by Western blotting. Lane 1 – WT mice, Lane 2 – WT + CS, Lane 3 – DJ-1 KO mice, Lane 4 – DJ-1 KO mice + CS. Relative expression is also shown. ( C ) p45 NFE2 expression (green) in ATII cells identified using SP-A antibody (violet) in lung tissue obtained from wild-type and DJ-1 KO mice by immunohistofluorescence. Cell nuclei were stained with DAPI (blue). ( D , E ) MMP9, CD147, cathepsin B, p45 NFE2 and ADAMTSL-4 mRNA expression in lung tissue from wild-type ( D ) and DJ-1 KO ( E ) mice by RT-PCR. The strongest p45 NFE2 expression is indicated by white arrows. *Statistically significant difference in comparison with control ( p < 0.05). Data are shown as the mean (±s.e.m.).

    Article Snippet: The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.

    Techniques: Expressing, Western Blot, Immunohistofluorescence, Staining, Reverse Transcription Polymerase Chain Reaction

    The role of p45 NFE in ATII cells in non-smokers, smokers and emphysema patients.

    Journal: Scientific Reports

    Article Title: The cytoprotective role of DJ-1 and p45 NFE2 against human primary alveolar type II cell injury and emphysema

    doi: 10.1038/s41598-018-21790-3

    Figure Lengend Snippet: The role of p45 NFE in ATII cells in non-smokers, smokers and emphysema patients.

    Article Snippet: The following antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA): MMP9, CD147, ADAMTSL-4, p45 NFE2, p18 NFE2, DJ-1, phosphoserine, phosphothreonine and phosphotyrosine.

    Techniques: